Unformatted text preview: hat allow
in vivo, real-time measurement of protein
expression, localization, modification and
activity/kinetics will be needed (Box 2). It
will be important to develop, refine and scale
up techniques that modulate gene expression,
such as conventional gene-knockout methods23, newer knock-down approaches24 and
small-molecule inhibitors25 to establish the
temporal and cellular expression pattern of
individual proteins and to determine the
functions of those proteins. This is a key first
step towards assigning all genes and their
products to functional pathways.
The ability to monitor all proteins in a cell
simultaneously would profoundly improve
our ability to understand protein pathways
and systems biology. A critical step towards
gaining a complete understanding of systems biology will be to take an accurate
census of the proteins present in particular
cell types under different physiological conditions. This is becoming possible in some
model systems, such as microorganisms26.
It will be a major challenge to catalogue
proteins present in low abun...
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This document was uploaded on 04/02/2014.
- Fall '14
- The Tempest