It will be important to develop refine and scale up

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Unformatted text preview: hat allow in vivo, real-time measurement of protein expression, localization, modification and activity/kinetics will be needed (Box 2). It will be important to develop, refine and scale up techniques that modulate gene expression, such as conventional gene-knockout methods23, newer knock-down approaches24 and small-molecule inhibitors25 to establish the temporal and cellular expression pattern of individual proteins and to determine the functions of those proteins. This is a key first step towards assigning all genes and their products to functional pathways. The ability to monitor all proteins in a cell simultaneously would profoundly improve our ability to understand protein pathways and systems biology. A critical step towards gaining a complete understanding of systems biology will be to take an accurate census of the proteins present in particular cell types under different physiological conditions. This is becoming possible in some model systems, such as microorganisms26. It will be a major challenge to catalogue proteins present in low abun...
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This document was uploaded on 04/02/2014.

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