chem160.problemset11.answers

Lineweaver burk plot 14 12 1v0 1 08 06 04

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Unformatted text preview: solutions Lineweaver- Burk plot Based on the equation for the Lineweaver- Burk plot, we know that the y- intercept corresponds to 1/Vmax, the slope corresponds to KM/Vmax, and the x- intercept corresponds to - 1/KM. Lineweaver- Burk plot 1.4 1.2 1/v0 1 0.8 0.6 0.4 0.2 0 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 1/[ATP]0 Performing linear regression on this plot yields the following information: y- intercept = 0.434 therefore, Vmax = 1/0.434 = 2.30 µM/s slope = 0.479 therefore, KM = 0.479*2.30 = 1.10 µM As a check on KM, we can calculate the x- intercept = - y- intercept/slope x- intercept = - 0.434/0.479 = - 0.907 therefore, KM = - 1/- 0.907 = 1.10 µM Chemistry 160 problem set 11 solutions Eadie- Hofstree plot Based on the equation for the Eadie- Hofstree plot, we know that the y- intercept corresponds to vmax, the slope corresponds to –KM, and the x- intercept corresponds to...
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