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Unformatted text preview: ns Eadie Hofstree plot (with competitive inhibitor present) • Vmax is unchanged so the y intercept stays the same. • KM is larger, which results in two things: o x intercept is smaller, as x intercept = Vmax/KM’ o slope is larger, as slope =  KM’, so the line is steeper. The red data points correspond to values reflecting the presence of inhibitor. The blue data points correspond to the original kinetic data in Problem 1. Eadie Hofstree plot 2.5 v0 2 1.5 1 0.5 0 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 v0/[ATP]0 Chemistry 160 problem set 11 solutions 3. The first plot is for a non competitive inhibitor, and, based on the parameters of the x and y axes, this is clearly an Eadie Hofstree plot. Thus, the parameters are as follows: • y intercept = Vmax’ • slope =  KM • x intercept = Vmax’/KM Removal of inhibitor (red squares) If the inh...
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This document was uploaded on 03/22/2014 for the course CHEM 160 at San Jose State University .
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