be230cLecture9

Aaron kaplan anp and phospholamban 18 16 14 12 1 anp

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Unformatted text preview: 0.8 0.6 0.4 0.2 0 Pacing Control Pacing 120 BPM Pacing 180BPM Paced 180 at 180 BPM X1 week UnPaced Unpaced Induced PleuripotentI V E S P E R S P E C T Cells Di ffe ? re n tia tio n ES cell Reprogramming Differentiation Shinya Yamanaka Somatic cell iPS cell Mouse gene combinations for iPS induction Oct3/4, Sox2, c-Myc, Klf4 Oct3/4, Sox2, Klf4 Oct3/4, Sox2, N-Myc, Klf4 Oct3/4, Sox2, Lin28, Nanog Figure 1 | Reprogramming between differentiNature Reviews | Molecular Cell Biology ated and default epigenetic states. The pluripotent cells of the early embryo give rise to all types of somatic cells, which are characterized by distinct epigenetic states. Differentiated somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by the introduction of a defined and limited set of transcription factors. Four combinations have been used successfully: modified cells fac hurdles for therap Researchers ha challenges. Two g morphological cr iPS cells without marker8,21. For hu expression patter markers for plurip have now reporte tial for producing result of this and that it is reasonab technical challeng near future. The m would be to achie gramming using o of the relevant fac any oncogenes. Human ES-cel icant differences i for specific lineag these lineage-spec for the establishm contrast, iPS cells possibility that the completely directe lineage. However 32 yo male resuscitated sudden death Echo: LV non compaction 32 yo male resuscitated sudden death Echo: LV non compaction 7 nml echo 4 nml echo 9 nml echo 3 nml echo 32 yo male resuscitated sudden death Echo: LV non compaction 7 nml echo 4 nml echo 9 nml echo 3 nml echo 32 yo male resuscitated sudden death Echo: LV non compaction 7 nml echo 4 nml echo Treated with3 nml 9 nml echo Defibrillator echo Model of Human Disease Clinical Threshold Gene A Sibling 1 Gene A Sibling 2 Model of Human Disease Clinical Threshold Environmental Insult Gene A Sibling 1 Gene A Sibling 2 Model of Human Disease Clinical Threshold Environmental Insult Gene B Gene A Sibling 1 Gene A Sibling 2 Model of Human Disease Environmental Insult Environmental Insult Gene B Gene A Sibling 1 Gene A Sibling 2 Clinical Threshold Can IPS Cells be used as a model of disease? What diseases? • Inherited Diseases • Cell Autonomous • Phenotype Detectable in Vitro iPS derived cardiomyocytes from Patients wit...
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