102 mm this volume will be used in part iii of the

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Unformatted text preview: ay assume that the samples were measured in a 1 cm cell): Sample Concentration, mM Absorbance 1 0 0 2 0.10 0.067 3 0.25 0.167 4 0.40 0.267 5 0.60 0.400 6 0.75 0.500 The equilibrium constant for an enzyme- catalyzed reaction is 1.54 x 10- 2 when 0.010 mol of enzyme are added to the reaction mixture. What changes would you expect in the equilibrium constant if 0.100 mol of enzyme were mistakenly added to the reaction? What changes would you expect if the initial amounts of reactants were doubled? Halved? What if the reaction was performed at a considerable higher temperature? Define chromophore. What solution(s) will the blank contain? Determine the concentration of NAD+ if 800 µL of 0.29 mM NAD+ solution is diluted to a final volume of 1700 µL. Determine the volume of 45 mM ethanol needed to make 1700 µL of a solution that has a final ethanol concentration of 26.5 mM. (This volume will be used in Part III of the experiment) Determine the volume of 0.29 mM NAD+ needed to make 1700 µL of a solution that has a final NAD+ concentration of 0.102 mM. (This volume will be used in Part III of the experiment) MATERIALS & METHODS Work in teams of four Note: NADH is prohibitively expensive for use in this lab. However, the chromophore in NADPH is the same as in NADH, and is cheaper and available. (Refer to your lecture notes for the structures) Part I: Determination of the Molar Absorptivity of NADPH 1. Obtain a stock solution of NADPH (~6.0 x 10- 4 M). Record the actual concentrati...
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This lab report was uploaded on 04/06/2014 for the course CHEM 272 taught by Professor Dr.brooks during the Summer '08 term at Maryland.

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