Lab 2 Measurement of the DNA Content of Strawnerries

Lab 2 Measurement of the DNA Content of Strawnerries -...

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Title: Isolation of DNA, Measurement of the DNA Content of Strawberries Introduction and General Objectives: DNA is the most studied topic in Biology. It has been studied many years, and only recently have there been major breakthroughs in understanding more and more about it. DNA is very long strands of nucleic acids that encode all of our body’s physical characteristics inside of it. To fit into proteins and cells, the DNA twists itself into compact units we call chromosomes. Today in this experiment, the objective will be to isolate the DNA of a strawberry with detergent, and sodium chloride. Also, we will measure the amount of DNA present in mg/mL by using absorbency measurements with a colorimetric assay. Hypothesis: Our hypothesis for this lab is that the absorbency will increase when the concentration of strawberry tissue is increased because when there is more tissue present, the more DNA is present as well. Materials and Methods: The materials we used were 10g strawberry, detergent, isopropanol, 2M NaCl, diphenylamine reagent, test tubes, beakers, and a spectrophotometer. The first step we did was make the solutions of the different concentrations of DNA because these must be heated in a water bath for at least 15 minutes. We prepared the solutions of DNA, NaCl, and diphenylamine. The concentrations were as follows: Desired Final Concetration ml of Stock DNA ml of NaCl ml of dyphenalamine
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This note was uploaded on 04/10/2008 for the course BSC 197 taught by Professor Nichols during the Fall '07 term at Illinois State.

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Lab 2 Measurement of the DNA Content of Strawnerries -...

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