Properties of Enzymes
Introduction and General Objectives:
In this lab, we investigated the effects on the reaction
rate when the enzyme catalase is introduced of H
--- > H
0 (l) + 0
When catalase is
introduced to the hydrogen peroxide, it breaks the H
down through phagocytosis.
were no way to break down hydrogen peroxide it would damage cell life inside of organisms
is a very strong oxidizing reagent.
The objectives of this lab are to study the effect of 5 different factors introduced to the
The control will be the 100% Concentration of catalase.
The five factors are: the
concentration of catalase, different pH levels, temperature, addition of inhibitors, and addition of
Because these five elements have a near same effect on all enzymes, this experiment
should give us a good insight into what they do to all enzymes.
The five different subsets in this experiment all must have different hypotheses.
For the effects of concentration, when the concentration of catalase is decreased, the reaction rate
will decrease as well and it will take the disc longer to float to the top.
For the effects of
temperature, the catalase that was boiled in the water bath should not produce any reaction
because it would have been denatured in the hot water bath and would be inactive.
catalase will react the same, maybe even more quickly than the room temperature catalase.
the effects of the levels of pH, when the pH level is closer to a base (pH 7 or higher) the reaction
rate will be faster and it will take less time for the disc to float to the top.
For the effects of
inhibitors, the catalase will not affect the reaction and we will not see the disc float to the top of
the hydrogen peroxide.
For the effects of the protease and the catalase, the catalase will be
inhibited by the trypsin and we will not see a reaction.
Materials and Methods:
In this experiment we used the potato catalase extract, filter paper
discs, forceps, a stopwatch, phosphate buffer, pH 3, 5, 7, & 9 solutions, trypsin, sodium azide,
and hydrogen peroxide.
For each of the five subset experiments we set up the appropriate
catalase concentration and added any necessary elements.
The first catalase we prepared was the
enzyme catalase and the lysate because it is required to incubate for an hour or more.