Lab 4 Properties of Enzymes

Lab 4 Properties of Enzymes - Title: Properties of Enzymes...

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Title: Properties of Enzymes Introduction and General Objectives: In this lab, we investigated the effects on the reaction rate when the enzyme catalase is introduced of H 2 O 2 --- > H 2 0 (l) + 0 2 (g). When catalase is introduced to the hydrogen peroxide, it breaks the H 2 O 2 down through phagocytosis. If there were no way to break down hydrogen peroxide it would damage cell life inside of organisms because H 2 O 2 is a very strong oxidizing reagent. The objectives of this lab are to study the effect of 5 different factors introduced to the catalase. The control will be the 100% Concentration of catalase. The five factors are: the concentration of catalase, different pH levels, temperature, addition of inhibitors, and addition of a protease. Because these five elements have a near same effect on all enzymes, this experiment should give us a good insight into what they do to all enzymes. Hypothesis: The five different subsets in this experiment all must have different hypotheses. For the effects of concentration, when the concentration of catalase is decreased, the reaction rate will decrease as well and it will take the disc longer to float to the top. For the effects of temperature, the catalase that was boiled in the water bath should not produce any reaction because it would have been denatured in the hot water bath and would be inactive. The chilled catalase will react the same, maybe even more quickly than the room temperature catalase. For the effects of the levels of pH, when the pH level is closer to a base (pH 7 or higher) the reaction rate will be faster and it will take less time for the disc to float to the top. For the effects of inhibitors, the catalase will not affect the reaction and we will not see the disc float to the top of the hydrogen peroxide. For the effects of the protease and the catalase, the catalase will be inhibited by the trypsin and we will not see a reaction. Materials and Methods: In this experiment we used the potato catalase extract, filter paper discs, forceps, a stopwatch, phosphate buffer, pH 3, 5, 7, & 9 solutions, trypsin, sodium azide, and hydrogen peroxide. For each of the five subset experiments we set up the appropriate catalase concentration and added any necessary elements. The first catalase we prepared was the enzyme catalase and the lysate because it is required to incubate for an hour or more. After we
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This note was uploaded on 04/10/2008 for the course BSC 197 taught by Professor Nichols during the Fall '07 term at Illinois State.

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Lab 4 Properties of Enzymes - Title: Properties of Enzymes...

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