Chapter 11 - Transcription and Translation

Within tfiid binds the tata sequence after tfiid

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Unformatted text preview: nce After TFIID binding, the rest of preinitiation complex assembles 3 o o o o o o o o o o o o 1)TBP is a subunit of the TFIID and when it binds to the promoter causes a conformation change in DNA 2) TFIIB binding site for RNA polymerase II with its attached TFIIF 3) After RNAPol-TFIIF is in position TFIIE and TFIIH join and transform the polymerase into an active, transcribing machine TFIIH functions as a protein kinase it phosphorylates RNA polymerase TFIIH also functions as a DNA helicase unwinds DNA; DNA must be separated to allow RNA polymerase access to template strand Transcription by RNA polymerase II can continue for hundreds or thousands of nucleotides beyond the coding sequences RNA then cleaved by a complex that associates with the polymerase seems to occur with termination of transcription mRNAS are polyadenylated at the 3 end poly A tail this process allows cleavage and also occurs with termination; both termination and polyadenylation use the same consensus sequence exact relationship between cleavage and termination remains to be determined cleavage itself could trigger termination polymerase activity could be agffected when passing through the consensus sequence at the cleavage site causes termination Eukaryotic mRNA Processing o o o o o o mRNAs share certain properties they each code for a specific polypeptide they are found in the cytoplasm they are attached to ribosomes when translated most have a non coding segment eukaryotic mRNA have modifitc...
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This document was uploaded on 04/10/2014.

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