Lecture 12 - Lecture #12- Recombinant DNA Technology and...

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Lecture #12- Recombinant DNA Technology and Molecular Techniques (chapter 13 p. 294- 311) I. Molecular Cloning Overview Cloning = making identical copies A. The process (Handout of Molecular Cloning Overview, figure 13.11) Tools: Steps: B. Uses -Sequencing -Transformation of organisms -Identification -Study of the gene's function -Identification and recovery of a single gene from a large collection of genes . -Testing for the presence of a genetic disease. -Study of the protein product -Production of the protein product for therapeutic uses (link, table 14.1)
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II. A closer look at the molecular cloning process A. Restriction Enzymes 1. Discovered by Smith and Nathans (1978) as enzymes produced by bacteria that cut up DNA of invading viruses. 2. Cut both strands of DNA at specific palindromic sequences (figure 13.6). Results in forming Sticky Ends = regions that extend from the end of the DNA as a single strand. 3. The sticky ends can base-pair (hybridize) with complementary nucleotides
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This note was uploaded on 04/09/2008 for the course BME 80H taught by Professor Rothwell during the Winter '07 term at University of California, Santa Cruz.

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Lecture 12 - Lecture #12- Recombinant DNA Technology and...

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