Analysis of Lambda DNA - Tiffany Clark Molecular Lab Analysis of Lambda DNA Introductory Information DNA cutting can be completed by restriction enzymes

Analysis of Lambda DNA - Tiffany Clark Molecular Lab...

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Tiffany Clark Molecular Lab 4-7-14 Analysis of Lambda DNA Introductory Information DNA cutting can be completed by restriction enzymes. These enzymes come from bacteria and used to cut invading DNA and are also used to cut digest DNA. These enzymes cut DNA at specific sites among the sequences of bases. Many restriction sites are palindromic. Lambda DNA is harmless to humans and comes from bacteriophage. The fragments cut by the enzymes can be analyzed by gel electrophoresis. The fragment sizes of HindIII when it digests lambda DNA are known, therefore this information is used to determine the unknown fragment sizes of EcoR1 and Pst1 digests. Prediction of what the HindIII lane will look like: **In my make-up lab section we ran out of Pstl digest. Methods Preparing the gel 1. 0.5 g of agarose was weighed out and placed into a flask with 50 mL of 1x TAE buffer. It was swirled to mix and then covered with a paper towel. The flask and solution was microwaved for 70 seconds total until the agarose was dissolved.
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