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Tiffany ClarkMolecular Lab4-7-14Analysis of Lambda DNAIntroductory InformationDNA cutting can be completed by restriction enzymes. These enzymes come frombacteria and used to cut invading DNA and are also used to cut digest DNA. Theseenzymes cut DNA at specific sites among the sequences of bases. Many restriction sitesare palindromic.Lambda DNA is harmless to humans and comes from bacteriophage. Thefragments cut by the enzymes can be analyzed by gel electrophoresis. The fragment sizesof HindIII when it digests lambda DNA are known, therefore this information is used todetermine the unknown fragment sizes of EcoR1 and Pst1 digests.Prediction of what the HindIII lane will look like:**In my make-up lab section we ran out of Pstl digest.MethodsPreparing the gel1.0.5 g of agarose was weighed out and placed into a flask with 50 mL of 1x TAEbuffer. It was swirled to mix and then covered with a paper towel. The flask andsolution was microwaved for 70 seconds total until the agarose was dissolved.