Cell Tissue and Water Relations LAB 3 ALEXIA.docx - Cell...

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Cell Tissue and Water RelationsAlexia KellyBiology 365: Plant PhysiologyDate Performed: January 28, 2021Date Due: February 4, 2021Burman UniversityPresented to: Dr. Buttler
AbstractThe observation made in the lab regarded turgor pressure, the movement ofwater and membrane permeability were seen in this experiment. Water potentialis the potential energy of water per unit volume that will then travel through thegradient until concentration is equal. Higher to lower, on either side of themembrane. ABA, a hormone that regulates aspects of plant growth, in this lab wefocus on the increase of membrane permeability and water movement. Fivepotato discs were taken and placed in four beakers containing a treatmentsolution including Water, ABA, ABA + Mannitol and Mannitol. Over a 90-minute period, at 15-min intervals, they were blotted, dry and weighed. Theywere weighed, then returned to their respective solutions. Mass changesindicated the rates of water flow in and out of the discs. Values were listed in atable and a graph was created to visualize the water movement rate through thechanging mass measurements. The results proved that the theoreticalassumptions made did not align with the experimental results. The effects ofABA were noticed on the discs, increasing the membrane permeability, andincreasing water movement. For the values documented did not match, theexperiment was conducted again, it would be improved from the human errorthen allowed for an in-depth understanding if the terms water potential,membrane permeability and turgor pressure.IntroductionTurgor pressure is the cause of fluid in a cell that presses and applies pressure on the cellplasma membrane against the cell wall, it causes formation of the plant. A wilted plant, that haslost its formation is most likely attributed to loss or lessening of turgor pressure. The rigidity of

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