ASW_p19 Final Lab Report - Inhibition of GATA-4 During the Differentiation of p19 Embryonic Stem Cells into Cardiomyocytes Alexis Weston Abstract The

ASW_p19 Final Lab Report - Inhibition of GATA-4 During the...

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Inhibition of GATA-4 During the Differentiation of p19 Embryonic Stem Cells into Cardiomyocytes Alexis Weston Abstract The potential benefits of stem cell research could provide major breakthroughs throughout the world of science. However, before these studies can move forward, it the job of researchers to understand how these cells can differentiate into such a large variety of different cells types. The purpose of this study was to further explore the MEK1-pathway activation of transcription factor, GATA-4. Here, we use a MEK1 inhibitor (U0126) to inhibit the phosphorylation of GATA-4, and further the differentiation of cardiomyocyte cells. We sought to determine the importance of GATA-4 in p19 embryonic stem cell differentiation to cardiomyocytes. Due to a lack of time and improper PCR technique, no tangible results were obtained. Further studies should be done to answer the research question. Introduction Stem cell research has been a hot topic in the scientific community for quite a few years. It’s no wonder, as these pluripotent cells can act as building blocks to a variety of different tissues necessary for survival in most organisms (NIH 2002). Questions have been asked as to whether or not these cells could become a way of meeting the needs of the ever-growing transplant list. It has been estimated that, on any given day, there are at least 3,000 people in the United States alone that are on the waiting list for a heart transplant (NHLBI 2009). In an ideal world, scientists could make organs from stem cells as needed and all of these issues would be solved, but we haven’t reached that point yet. Before we can even consider attempting these
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types of novel experiments in humans, we have to first use an animal model to fully understand the ins and outs of how stem cells function. The differentiation of these cells into other various cell lines is entirely dependent on which signals they receive and at what time during development they receive them (NIH 2002). There are many different factors that affect the overall differentiation of cells and understanding them all can become a rather large task. Our experiment works to better understand p19 embryonic mouse stem cells and the signals that stop and start their differentiation into cardiac muscle cells. For the purposes of our experiment, we decided to focus on GATA-4, a
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  • Spring '14
  • Avery
  • Genetics, Cellular differentiation, Embryonic stem cell, Embryonic stem cells

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