Stem Cell Research Proposal - Inhibition of GATA-4 in the Differentiation of p19 Embryonic Stem Cells into Cardiomycocytes Alexis Weston Margaux Pugh

Stem Cell Research Proposal - Inhibition of GATA-4 in the...

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Inhibition of GATA-4 in the Differentiation of p19 Embryonic Stem Cells into Cardiomycocytes Alexis Weston, Margaux Pugh, Nick Cramer, Somi Yoo Introduction: Stem cell research is an increasingly important subject in medical research and genetics. With the growing shortage of organs for transplant and the potential that lies in stem cell research for repair of bodily damage and sickness, it is easy to see why this area of research is the focus of many. Stem cells are the basis of all other cells in the body and are able to differentiate depending on their environment and the signals they receive. There are many different factors that affect the overall differentiation of cells. There have been many examples of research in this area, but our experiment focuses on the use of p19 embryonic mouse stem cells. Over the years scientists have been able to differentiate these cells into nearly every type of cell found in humans, but the mechanisms by which this occurs are not entirely understood. For the purposes of our experiment, we have decided to focus on GATA-4, a specific gene thought to be vital for the differentiation of p19 cells into cardiomyocytes. The goal of our experiment is to determine if differentiation to cardiomyocytes is possible with the inhibition of GATA-4. In past experiments, scientists have used many different chemicals and conditions just to differentiate p19 cells into cardiac cells. In some, they even used concentrated GATA-4, which is an enzyme that is actually produced from cardiac cells. Even when treated with other chemicals to induce cardiac differentiation, GATA-4 is expressed. This GATA-4 is a gene only expressed in cardiac cells, which is transcribed and translated into the enzyme that also contributes in
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differentiating the cells into cardiac cells (Grepin 1995). For this reason, our purpose of the
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  • Spring '14
  • Avery
  • Genetics, Cellular differentiation, Embryonic stem cell, Embryonic stem cells, Cell culture

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