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Lab 4 NotebookBack to Home PageTitle: UF04 Growth Media Objective: To understand the types and uses of growth media for the isolation and identificationof unknown bacterial samples Procedure: 4-phase dilution streaking: Clonal isolation Using a sterile loop spread culture in area # 1 x1 Using a New sterile loop drag through the end of area 2 x 1Use a new sterile loop drag through the end of area 3 x1oUse back and forth pattern to dilute the culture in each zone oInvert plate and incubate overnight @ 37degree Celsius Procedure: non-selective agarQuadrant growth: rapid test for multiple isolatesUse sterile loop spread unknown culture A in area #1Use a new sterile loop spread unknown culture B in area #2Use a new sterile loop spread unknown culture C in area #3Use a new sterile loop spread unknown culture D in area #4oUse back and forth pattern to dilute the culture in each zone oInvert plate and incubate overnight @ 37degree Celsius

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