Midterm Review - BIOL334Midtermexam...

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BIOL334  Mid-term exam Catalytic efficiency  is Kcat = turnover # = Vmax/amt of enz in umol Q10  is the temperature coefficient of a chem rxn after you increase the  temperature by ten degrees; Q10 = V(TC + 10C) / V(TC); usually equal to 2.0 for  mammals In vivo  is part of a living organism,  in vitro  is when a part has been isolated At equilibrium, dG0’ = -RT ln Keq dG’ = dG0’ + RT ln ([B]/[A]) keep in mind that activation energy is separate from dG; a reaction can be very  exothermic but not go through because its Ea is not reached mass action ratio  = [p]/[s] If dG = 0 or if mass action ratio = Keq, then the enzyme is freely reversible and  close to equilibrium If dG << 0 or Keq >> mass action ratio, then it is not freely reversible, mostly like the substrate of a pacemaker enzyme Vmax  is the max catalytic rate, when enzyme is saturated with S Kcat  is the turnerover # = Vmax/amt of enz Km  = michaelis constant = [S] yielding 0.5 Vmax (indicates enz affinity for  substrate; low Km value means enz has high affinity for its S S0.5  is [S] required for 0.5Vmax, where S means signoidal Nh  hill coefficient = degree of copperativity of S binding; n = 1 means the enz is  not reliant on coop, n >> 1 means enz is very reliant on coop (sigmoidal) Catalytic efficiency Ki
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