Unformatted text preview: Terms describing cells with (and without plasmids} “F” = fertility plasmid (an example of a conjugative plasmid found and Studied in
Escherichia coli) L - 1Ir'n-v‘: -..' i' 1- -"--\-l'i"‘l'1l"-'.<Ix F+= it 37:1";- [.4 a : "l'i" ' " - ' Fi": L t l t P -._1-
l l-V a . F ll: as: t i. ~ ‘ ' l ‘ (Hfr stands for "high frequency of recombination”) Figure 10.22a Chromosomal DNA may also be transferred in conjugation: Some plasmids may become integrated into chromosome. These plasmids are called -AL?L—h— The cells are designated as ti 7 _ These integrated plasmids may mobilize (or facilitate the transfer of) the
chromosome during Conjugation. They can transfer a large region of
chromosome. Example of such a plasmid:
if l"'. , 'i LL. It: Hfr cell-now serves as F plasmid donor to F cells (those lacking an F plasmid)
and some chromosomal DNA can also be transferred. (If an F+ cell is the donor,
then "no chromoSomal DNA can be transferred; only plasmid.) See‘iﬁgare lighter how an Hfr cell can transfer plasmid and chromosome to an
F cell. Reminder about ctmjugation: . .-. . . :1 ,' 9--
n L .1- l l“"- 'I 1.1! ‘ 1L ‘kl "' l1“ .
I' 'u r1 _ A. . '
'r l \ -:.|.l.- ...
View Full Document
- Fall '07