Microbial Growth Under Varying Conditions

Microbial Growth Under Varying Conditions - Daniel Morgan...

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Daniel Morgan Microbial Growth Under Varying Conditions The ability of the bacterium Escherichia coli (E. coli) to grow and indeed prosper in any given environment is contingent upon the makeup of the ecosystem, primarily its nutrient composition, temperature, pH, aeration, salt concentration and osmotic pressure. While all these aspects play a large role in determining the prosperity of the bacterium, we chose to focus on the three components of primary concern of said environment: the amount of aeration, the amount and variation of nutrients available to the bacterium, and the temperature. When first introduced to a new medium with an environmental differing from that of its previous, the bacterium takes time to acclimate itself to its newly found surroundings, known as the lag period, in which time lipids and enzymes are synthesized. This increase in activity actually increases the cell’s size, leading to its division and the initiation of the log phase, in which exponential growth of the bacteria colony is witnessed. After this growth and division has consumed all the available resources necessary to carry on such processes, a stationary phase follows, in which as many bacterium are made as those that die of lack of nutrients. As is the case for all organisms, the death phase finished the process due to an inability to sustain such a population as has been accumulated (Herendeen, S.L). This process is known as the Mean Generation Time of, in this case, the E. coli bacterium, and is regulated fully by the factors aforementioned, three of whose impact will be tested during this lab. A bacterial culture was placed in a number of flasks with which to test the two degrees of aeration, the three of nutrient availability, and three temperatures. I suspect the highest growth will be observed in the baffled flask (due to extra oxygen availability to all the cells, Berney, Michael), the flask induced with MSGTYE, a combination of salt medium with glucose, tryptose, and yeast extract (due to all the same nutrients as are present in the other flasks with the
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Daniel Morgan addition of the yeast extract), and the 37 ° C flask (being exposed to higher temperatures than would normally be experienced by this human affiliated microbe, and therefore more energy is available). Methods
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Microbial Growth Under Varying Conditions - Daniel Morgan...

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