New Material for BIO 110 FINAL

New Material for BIO 110 FINAL - New Material for BIO 110...

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New Material for BIO 110 FINAL DNA Scientists Frederick Griffith o Discovers bacterial transformation o S strain has cell-wall like capsule that keeps virus from being attacked in mouse; R strain doesn’t o Living S strain kills mouse; living R strain results in healthy mouse o Heated S strain results in healthy mouse o Mixture of heated S strain and R strain results in dead mouse o Concluded: The living substance still present in the heated S strain must be genetic material Avery, McCarty, and MacLeod o Used different enzymes to degrade different components in heated/killed S strain o Enzymes: o Transforming agent is not carbohydrates, lipids, RNA, proteins; Transforming agent is DNA o Concluded: DNA must be transforming agent Hershey and Chase o Preformed “Blender Experiment” with T2 Bacteriophage o Protein tagged with S-35; DNA tagged with P-32 o Phage attached to E. coli and injects viral substance o Protein (S-35) does not enter E. coli; DNA (P-32) enters E. coli o Concluded: DNA, not protein, is the genetic material that phage uses to make new virus particles Watson and Crick o Took information from others and put together structure of DNA o Building blocks of DNA are nucleotides Bases = purines are Adenine (A) and Guanine (G) ; pyrimidines are Cytosine (C) and Thymine (T) Sugars = deoxyribose 1
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Phosphate o DNA structure in right-handed and antiparallel o A and T / C and G are paired by creating hydrogen bounds Base Pairing DNA Base Pairing: o A = T ; G = C RNA Base Pairing: o T = A ; A = U ; G = C ; C = G Codons The initiation (start codon) of translation is AUG The termination (stop condons) of translation are UAA, UAG, UGA Codons form the correct codes in triples (“Triplet Condons”)
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This note was uploaded on 04/07/2008 for the course BIO 110 taught by Professor Fairchild during the Fall '07 term at West Chester.

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New Material for BIO 110 FINAL - New Material for BIO 110...

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