Determination of Quinine in Tonic Water by Fluorescence Spectrophotometry

Determination of Quinine in Tonic Water by Fluorescence Spectrophotometry

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Determination of Quinine in Tonic Water by Fluorescence  Spectrophotometry
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Abstract: The object of this lab is to determine the concentration of quinine in tonic  water, using fluorescence spectrophotometry. Fluorescence is the emission of a  photon at different wavelengths. There is normally a specific relationship  between the intensity of the emitted radiation and the sample concentration over  some specific concentration range (Beer’s Law is obeyed). Using the  spectrophotometer, concentrations of 0.00, 0.01, 0.1, 1.0, 5.0, 10.0, 25.0, and  50.0 ppm were found to have intensities of 0, 0.087, 0.795, 7.159, 34.793,  67.365, 246.319, and 340.619 respectively. The intensity of the unknown was  measured to be 249.145. Using (Concentration = k1 * I + k0), where k1 = 0.138  and k0 = 0.613, we found that the concentration of unknown = 34.99501 ppm.  Converting to molarity, our unknown = 1.0787 M.
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Determination of Quinine in Tonic Water by Fluorescence Spectrophotometry

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