Plants.2.08 - 0 NOTE! These files are provided for the sole...

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0 NOTE!! These files are provided for the sole purpose of assisting BSci 110 students to study for exams in the class. Some of the material in these files may be copyrighted, and it is not OK for you to share these files with anyone who is not a student in this class or to use them for any purpose other than to study for the exams of our class. Thanks, Carl Johnson
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Plants Almost all life on earth is based on plants, because they are solar-powered and trap the sun's energy for other life to use. They produce the oxygen we breathe, and transformed the atmosphere of Earth, making aerobic metabolism possible. 1
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Plants What is the most abundant protein on earth? Purves 8.3 The “solar-power” comes from photosynthesis, which occurs in chloroplasts. 2
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Plants Purves 8.3 Rubisco !!!! (fixes carbon in the Calvin Cycle of photosynthesis–Rubisco constitutes 50% of all protein in the leaf) And, thylakoid membranes are the most abundant membranes on earth! Purves 8.14 3
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Plants Karp 8.36 Purves 15.17 Unique/unusual features of plant cells: cell walls, chloroplasts (cpDNA), large vacuoles, plasmodesmata (syncytium), polyploidy, self-fertilization possible (but not preferred). 4
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Plants: non-food uses Cotton 5
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Corn Rice Soybeans Plants as Food Wheat Potatoes 6
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Purves Table 16.2 Plants: the "Green Revolution" Genetic therapy has not yet worked well in humans, but the creation of transgenic plants has been successful and is big business (but controversial). 7
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Karp 18.45 Making Transgenic Plants Transgenes are inserted into plasmids that can be transferred to the plant by infection with the bacterium Agrobacterium . Usually an antibiotic resistance (kanamycin or gentamycin) is linked to the transgene of interest to select for transformed cells. Transformation is done on shoot or leaf tissue that forms a callus when grown on agar medium. 8
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Making transgenic plants works so well because the infection with Agrobacterium is efficient and because entire plants of many species can be regenerated from calli tissue (we mentioned this before in the context of totipotency). Calli grown under different concentrations
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This note was uploaded on 04/07/2008 for the course BSCI 110B taught by Professor Due during the Spring '08 term at Vanderbilt.

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Plants.2.08 - 0 NOTE! These files are provided for the sole...

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