2004exam-young - Midterm exam #1 Ted Young Total points...

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Midterm exam #1 Winter quarter 2004 KEY Ted Young Total points 100. Part I. Multiple choice. (2 points each; total 40 points 1. Which polymerase subunit is responsible for specific initiation of transcription at a eukaryotic promoter? A. β B. α C. β D. σ E. none of the above 2. The carboxy-terminal domain (CTD) of RNAPII is reversibly modified during the transcription cycle by which of the following? A. specific transcription factors B. Acetyl transferases and deacetylases C. Kinases and phosphatases D. hydrogenases and dehydrogenases E. more than one of the above 3. Which of the following techniques could be used to determine the physical position of a promoter sequence A) ribonuclease analysis B) S1 nuclease analysis C) footprinting D) northern hybridization E) none of the above 4. YAC vectors A) carry a yeast selectable marker (gene ) B) must be packaged using a lambda packaging mix C) replicate in yeast as a circular plasmid D) can only carry fragments on the order of 10kb to 30kb E) none of the above 5. The S1 nuclease assay is used to A) Determine the 3’ end of an RNA with respect to the DNA sequence. B) Determine the 5’ end of an RNA with respect to the DNA sequence. C) Determine which DNA strand is transcribed. D) All of the above
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6. When setting up a PCR reaction with two primers of 20 nucleotides each, which of the following consideratins determines the temperature of the annealing reaction: A. The Tm of the DNA product B. The Tm of the oligo with the highest (G+C) content C. The Tm of the oligo with the lowest (G+C) content D. The termperature sensitivity of the DNA polymerase 7. All the following except (blank) are required for PCR A. DNA polymerase B. Primers C. DNA ligase D> dNTPs E. All of these are required for PCR 8. Nucleosomes A. Help condense DNA B. Are composed of the proteins H2A, H2B, H3 and H4 C. Play an important role in the regulation of gene expression D. All of the above 9. Which primer would have the highest Tm? A. AAGAATTC B. CCAGCTGG C. TCAAGACC D. CCTTTAGC E. Cannot be determined 10. General transcription factors A. Bind to enhancer elements B. Are important in codon recognition C. Are required for transcription initiation D. Consist of both RNA and proteins E. All of the above 11. Modifications to histones A. Occur mainly in the core of nucleosomes B. Don't change the net charge of the histones C. Influence the accessibility of DNA to transcription D. Are irreversible E. All of the above 12. Splicing in Eukaryotes
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A. Allows the production of different protein products from the same gene B. Is dependent on ATP C. Can remove exons from pre-mRNA D. Can remove introns from pre-mRNA E. All of the above 13. The primosome consists of: a. DNA polymerase and DNA primase. b
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2004exam-young - Midterm exam #1 Ted Young Total points...

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