Column and Thin Layer Chromatography

Column and Thin Layer Chromatography - Column and...

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Column and Thin-Layer Chromatography of Spinach Extracts Jacob Rietschy Chemistry 330 Nate Ware
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Introduciton: Spinach extract was separated into its individual components using column chromatography. Thin-layer chromatography was also used with spinach extract for analytical purposes. The results of the two chromatography procedures were compared. The spinach was separated into its components with column chromatography. Conclusions were made about the polarity, purity and identity of the spinach extract. Reactions/Mechanisms/Theory: Chromatography is a process used for separating and purifying both solid and liquid compounds. The three types of chromatography are column, thin-layer and gas chromatography. In column chromatography, a solid adsorbent is eluted with a liquid. In this sense, the solid acts as the stationary phase and the liquid acts as the mobile phase. The solid is usually either alumina or silica gel, with alumina being basic and silica gel acidic. The adsorbent is supported by a glass tube with a stopcock. In the stopcock is a wad of cotton and sand to keep everything in the column. Alumina and petroleum ether are above the sand and cotton in the column and a layer of sand is on top of that. The solvent should be above the sand and not below it for the column can not be allowed to dry. The solvent and spinach extract pass through the adsorbent in the column. Thin-layer chromatography is used for identifying compounds and determining their purity. Silica gel plates are most commonly used for TLC. The silica gel powder is kept on the slide by the manufacturer adding plaster to the silica gel. The spinach extract solution is spotted on the TLC plate.
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This note was uploaded on 04/09/2008 for the course CHEM 0330 taught by Professor Forget during the Spring '07 term at Pittsburgh.

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Column and Thin Layer Chromatography - Column and...

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