Genome Organization

Genome Organization - Adair Bio 1306 Sources: Campbell,...

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Adair Bio 1306 Sources: Campbell, Biology, Molecular Biology of the Cell (Alberts et al), Purves, TW Genome Organization 1. Eukaryotic Chromosomes: A chromosome is a highly compact structure. (Review Purves p 169-170) a. What is chromatin? b. What are histones? c. What are nucleosomes? 2. The eukaryotic genome is larger and more complex that the prokaryotic genome. (Review Purves p 279-283) a. List 5 general differences seen between eukaryotic and prokaryotic genomes. b. How many proteins are found in E. coli? In yeast? c. What types of genes do yeast cells carry that prokaryotes do not? d. How many protein-coding genes are found in C. elegans? e. What type of genes are found in the C. elegans genome and not in yeast? f. Name at least three additional model organisms whose genomes have been sequenced. 3. Repetitive DNA and other noncoding sequences account for much of a eukaryotic genome . a. About 97-98 % of DNA in humans is noncoding. b. Tandemly Repetitive DNA: repeated in series i. Proportion in mammals: 10-15% ii. Length of each repeated unit varies. iii. Total length of repetitive DNA per site: 10 -10 million bp iv. What does “Satellite” refers to? v.
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Genome Organization - Adair Bio 1306 Sources: Campbell,...

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