Lecture20-2007 - History of Glycolysis Glycolysis I Lecture...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Glycolysis I Lecture 20 Chapter 14 Sections 1 and 2 History of Glycolysis • 1850’s: Pasteur showed yeast can ferment sugar to CO 2 and ethanol, but he cannot replicate with extracts, proposes “vitalism”. • 1898: Hans and Eduard Buchner demonstrate yeast extracts ferment sugar to CO 2 and ethanol, proving vitalism false. Birth of biochemistry. • 1905: Harden and Young dialyze yeast extract into two fractions: – Retained (high molecular weight), called “zymase”. – Dialyzed (low molecular weight), called “co-zymase”. – Need to mix together to recover ability to ferment sugar. – Lose activity if boil zymase, but not if boil co-zymase. Yeast Extract Zymase Co-zymase What were Zymase and Co- zymase? • Zymase: high molecular weight, heat- labile enzymes. • Co-zymase: low molecular weight, heat-stable factors: – Inorganic cofactors: Pi, Mg ++ – Organic coenzymes: ADP, NAD +
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 NAD + : N icotinamide A denine D inucleotide • Mobile 2 e - carrier. • Carries 2e - in the form of hydride ion: H + + 2e - => H - • Usually involved in oxidation of primary alcohol to carbonyl: R-CH 2 -OH + NAD + => R-CH=O + NADH + H + • Derived from niacin (vitamin b3) Overview of Glycolysis • Glycolysis means “sweet splitting” – Glyco: Greek for sweet – Lysis: Greek for splitting or loosening • Split a 6-carbon molecule (Glucose) to two 3-carbon molecules (pyruvate). • Largest carbon flux in most cells. • Best understood metabolic pathway.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/09/2008 for the course BIOCHEMIST 361 taught by Professor Haltiwanger during the Fall '08 term at SUNY Stony Brook.

Page1 / 14

Lecture20-2007 - History of Glycolysis Glycolysis I Lecture...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online