Lecture21-2007 - Balanced equation for Glycolysis: Glucose...

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1 Glycolysis II Lecture 21 Chapter 14, Sections 3 and 5 Balanced equation for Glycolysis: Glucose + 2 ADP + 2 Pi + 2 NAD + ------------> 2 Pyruvate + 2 ATP + 2 NADH + 2 H + + 2 H 2 O Glucose from food. ADP and Pi from constant breakdown of ATP. Where does NAD + come from? Requires O 2 and mitochondria Exercising muscle, RBC, some bacteria Yeast Fermentation: Regeneration of NAD + in the absence of O 2 or mitochondria Lactic acid fermentation: Lactate dehydrogenase Reversible reduction of pyruvate to L-lactate with NADH. Balanced equation for lactic acid fermentation: Glucose + 2 ATP + 2 Pi ---> 2 Lactate + 2 ATP + 2 H 2 O
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2 Ethanol Fermentation: 1. Decarboxylation of pyruvate to acetaldehyde. 2. Reduction of acetaldehyde to ethanol. TPP TPP can form a stable carbanion: TPP is non-covalently, but tightly, bound to enzyme Decarboxylation reactions proceed through an unstable carbanion intermediate: C - TPP stabilizes charge by resonance
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3 Balanced equation for Ethanol Fermentation: Glucose + 2 ADP + 2 Pi ---> 2 Ethanol + 2 ATP + 2 CO
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Lecture21-2007 - Balanced equation for Glycolysis: Glucose...

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