Metabolism - bra[Mmfiafifllflmmm East Mfr-ale...

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Unformatted text preview: bra [Mmfiafifllflmmm East Mfr-ale ming'flilp= catalysis} 515—.“ Hill wry-fl -.:’ muse-Willi)» m+ Ir-lllrl'i'elocllpfl’n! "5 “'20 IE”! Inm'l'ish WMWVP -J.§., IIII ENI mas Es complex. Lm'fium" ' :nmihua sdvadflmsra'filad are“: lien ”law «be whammnlnn lint-HI mmmflmwfl: kdfl'nllflllf'lf-l): M=fvmlammtdENZiupmnfl= [#ufS molecules converted to Pisec] permolecule nfEflZ, r IhenEllZissalun‘lJedu-Iilss ”eaten 8 Michaelis “firm = ”A (iii)deriuaddmirM-Mequa1iunm hmmmmwm-Lluflxrfimr relu[5]mi|’.o‘lanflflfilaiymiru\_ 'I'urrlwer Numbers {lrml of Same Enzymes V. = (V.- X {31] m Swarm "‘- + ”H Catalan Hang carbmic magma; H03: Vm=lhxgafilpfinm ='II|'. wimEflZismflr-iflls Mgwhminmrm Acmlcrwling Fur-1'1: "sully: lunnl Ppmdllnda'minflmgn‘ffilzllrubeh fl-lmmasq Benn-Ipcniullin Fumarase Fu ITIHIETE Rec! Dmluin tan filPaxll RTP D- H. = W:m=p}mmsv__ >- K. gives idea DfENZ'a ‘afinity'futfi > K, uniLI = mll [usually] _ . . Hum“, [or ijKn] = "mum: eflm‘mcf' of EHZ = unclul for comparing different ENZs ; units = ""396 '1 > K_ values are usually similar to lhe respeclive in viva [S] “we hymn-e "‘“E- “Bum“ Eula“ W “ :. ENZs rarer: saturated wilh their 5, in viva “ > all ENZs tend to differ in their um E Km = fixed constants 1w myENZ & what we want to know How to determine V...“ & Km ? Could estimate V...“ & K... from plot of 1]., vs. [5]... tullallrnlsfi'lrw° .flmlihflw amoral: “MORE COMPLEX FuHCTFOPI‘ of: mil {2.}. ENE alumna-ism} rEquiras IIDRE COUPLE)! it large-1. 'nafiw' STRUCTURE = I." swam“ mm Eflzm Knoll I255 onmrnon hulihese mfinfiinfltfilh (‘3 Only EDIE “at“ malfimeric [have 4’siruclure] can oahlyae this type of rm... damswmmm tin-I} Irma—y scandal-y 1m nan-nu.- mul- Ialmgw Quanta-am: Hum! [mmEllz [laws-INK =a-mlfilfl-ic' = monomeno' nah're EH2 Cooperative ENZs often contain 3-D sites where Inlliiitors [1:- Hor activators [A] out rte-mainly bind - this hiking promotes a :5 ENE! conformation A 14..., Not A K... amusing: all-5mm mums=aflalllriBEflZI mmfAun=Mlfllm EMMQII'lflwlfilF-l 'Hnnlnblpic'EflZ = Allosten'c modliator = S - realm in Sigmoidal l'oooperaiive'] 5 satiation m'm=flllostefic motlllalnr ‘9 S - remit: in either activation or illlihition of V. - note: my alloalerit: EH2: He m hnmoIro-pir. a interntropio ...
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