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BIO40-17 DNA replication HO

BIO40-17 DNA replication HO - 5’> 3’ exonuclease...

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DNA replication: Conservative, Semiconservative, or Dispersive? Matthew Meselson and Franklin Stahl (1958) => semoiconservative DNA replication in circular chromosome of bacteria John Cairns (1963) unidirectional or bidirectional? => bidrectional How many origins of replication? One or many? => One in prokaryotes DNA polymerase I First in vitro synthesis of DNA -> Authur Kornberg 1957 (Nober Prize 1959) DNA polymerase requirements: dNTPs, Template, Primer DNA polymerase I functions: 5’-> 3’ polymerase activity
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Unformatted text preview: 5’ -> 3’ exonuclease activity 3’ -> 5’ exonuclease activity P P OH OH 3' 5' 5' 3' 5'-3' exonuclease 3'-5' exonuclease 5'-3' polymerase DNA polymerases II and III in Prokaryotes DNA polymerase II: activity, functions, structure DNA polymerase III: activity, functions, structure DNA polymerases in Eukaryotes ! (or I), , , (or III); and (or II) functions Fidelity of DNA replication: one error in every billion base-pair replication. Proofreading => 3'- 5' exonuclease activity....
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