Lecture #23 - BioMI 290, Fall 2007, Lecture 23 1 Lecture 23...

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Unformatted text preview: BioMI 290, Fall 2007, Lecture 23 1 Lecture 23 Lecture 23 BBOM 11e Sec. 7.14 - 7.16 Translation (Protein Synthesis) Translation (Protein Synthesis) BBOM 11e Sec. 7.14 7.16 Translation: Role of The Ribosome Translation: Role of The Ribosome 50S: 23S+5S rRNA + 34 proteins 30S: 16S rRNA + 21 proteins The 70S Ribosome = mRNA + 30S+ tRNA-AA + 50S BioMI 290, Fall 2007, Lecture 23 2 The RBS is complementary to the 3-end of 16S rRNA The Ribosome Binding Site (RBS) In Prokaryotes The Ribosome Binding Site (RBS) In Prokaryotes Initiation: in the 30S subunit DNA mRNA Ribosome-binding site AGGA Adjacent START codon AUG mRNA tRNAs Are Adapter Molecules tRNAs Are Adapter Molecules Transfer RNA (tRNA) molecules bring amino acids to the ribosome. One or more types of tRNA molecules exist for each of the 20 amino acids. BioMI 290, Fall 2007, Lecture 23 3 Role of Aminoacyl-tRNA Synthetases Role of Aminoacyl-tRNA Synthetases To attach an amino acid to the correct tRNA Two Reactions: AA + ATP AA-AMP + PP i AA-AMP + tRNA tRNA-AA + AMP Initiation Initiation 2 GTPs are required for the initiation complex to form 1. Initiation complex: 30S ribosome, mRNA, f-Met-tRNA, initiation factors 2. 50S subunit is added to form the 70S ribosome BioMI 290, Fall 2007, Lecture 23 4 Elongation:The Peptidyltransferase Reaction Elongation:The Peptidyltransferase Reaction .. P site A site 1 GTP is required for translocation & release of tRNA as mRNA moves to next codon Proteins Note: there are Atomic Structure of 50S Ribosome Subunit Atomic Structure of 50S Ribosome Subunit Peptidyl transferase no proteins near the enzymatic site. The 23S The 23S rRNA rRNA is is therefore an therefore an n m n m En m s En m s 23s & 5s rRNA Atomic structure of 50s ribosomal subunit at 2.4 Angstroms Ban et al. 2000. Science 289:905-920 transferase site enyzme enyzme. Enzymes . Enzymes composed of RNA composed of RNA are sometimes are sometimes referred to as referred to as Ribozymes Ribozymes . BioMI 290, Fall 2007, Lecture 23 5 Now know both prokaryotes & eukaryotes Carl Woese hypothesized there was 1...
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Lecture #23 - BioMI 290, Fall 2007, Lecture 23 1 Lecture 23...

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