Lecture #16 - Lecture 16 Finish Microbial Ecology Methods...

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1 Lecture 16 Lecture 16 Biofilms BBOM 11e Sec. 19.1-19.3, 21.3 Microbial Ecosystems Microbial Ecosystems Virtual field trip to NSF Center For Biofilm Engineering, Montana State University, Bozeman Lichens Read: BBOM 11e Sec. 19.20 Finish Microbial Ecology Methods Finish Microbial Ecology Methods Enrichment and Isolation Culture Methods Enrichment and Isolation Culture Methods Winogradsky Column Selective Enrichment Cultures Extinction Dilution Cultures BBOM 11e Sec. 18.1-18.2
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2 Synthesize Fluorescent oligonucleotides for known and unknown sequences Community Analysis with the Polymerase Chain Reaction (PCR) Community Analysis with the Polymerase BBOM 11e Sec. 18.5 PCR primers: Short 16S DNA oligonucleotides to start the reaction Taq polymerase PAGE: Poly-acrylamide gel electrophoresis Multi-probe FISH Shows known and unknown organisms in different colors Compute a 16S rRNA tree The microbial community structure of compost Microbial Community Structure in Any Lake Microbial Community Structure Genetically related individuals = Populations work side-by side to cycle nutrients and maintain energy flow in an ecosystem Metabolically related populations that share the same resources = Microbial Guilds BBOM 11e Sec. 19.1 Guild 5 Oxic Zone Anoxic Zone
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3 Microbial Biofilms Microbial Biofilms What are biofilms ? Communities of microorganisms attached to a surface and encased by excreted heteropolysaccharide-protein polymers (glycocalyx) How do they form?
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This note was uploaded on 02/13/2008 for the course BIO 2900 taught by Professor Ghiorse during the Fall '07 term at Cornell University (Engineering School).

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Lecture #16 - Lecture 16 Finish Microbial Ecology Methods...

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