Lecture #14 - Lecture 14 Bacterial growth parameters...

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1 Lecture 14 Lecture 14 Physical and Chemical Factors Affecting Growth Physical and Chemical Factors Affecting Growth BBOM 11e Sec. 6.4-6.6 , 6.10-6.12, 12.4, 12.34, 13.3, 13.5, 19.14 Virtual Field Trip: Yellowstone National Park Temperature Temperature Bacterial growth parameters Quiz #2 Lectures 8-12, SGDI Bacterial Population Growth Parameters Bacterial Population Growth Parameters Binary Fission Model 1 cell 2 cells Semilogarithmic graph EXPONENTIAL PHASE N O N Measure this key parameter: n =number of doublings in time, T log N - log N O log 2 = n n = 7 - 6 0.301 = 3.3 doublings in 2.6 hours T =2.6 h Doubling time dt = T/n = 2.6/3.3 = 0.79 h During exponential growth phase: Growth rate (=Division rate) r = 1/dt = 1.27 doublings/h (= 47.4 min) BBOM 11e Sec. 6.1, 6.4-6.6
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2 The Top Five Environmental Factors The Top Five Environmental Factors Temperature Low and high pH Salinity (Water activity) O 2 concentration Radiation Physical and Chemical Factors Antiseptics Disinfectants Antibiotics Growth factor analogs Antimicrobial Chemical Agents Long-term evolutionary adaptations Recent development of genetic resistance Lecture 14 Lecture 15 Temperature Temperature Cardinal Temperatures for growth and survival T max T opt BBOM 11e Sec. 6.10 T min T max and T min
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