learning goals for lectures 35 and 36

Learning goals for - The endosymbiont hypothesis for the origin of mitochondria Early eukaryotes engulfed a purple bacteria that was capable of

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The endosymbiont hypothesis for the origin of mitochondria Early eukaryotes engulfed a purple bacteria that was capable of producing ATP and then it became the mitochondria organelle The general types of electron carriers and their distinguishing characteristics NADH and FADH2 NADH associates reversibly with DHs, carries electrons from catabolic reactions to their point of entry into the respiratory chain, takes 1 e- FADH2 tightly bound to DH so protein must move to transfer electrons, can accept 2 e- Iron-sulfur proteins transfer 1 e-, similar to heme structure with iron surrounded by ring to stabilize it Cytochromes contain heme and therefore absorb light, carry one e-, three classes: a, b, and c, the heme contains iron which carries the electron Ubiquinone also called coenzyme Q, lipid soluble because has long isoprene side chain, can accept one e- and one proton to become semiquinone radical, or two e- and two protons to become ubiquinol Order of electron carriers in mitochondria; how can this order by determined experimentally? Order of reduction potentials going from lower to higher Carrier Standard reduction potential NADH -.32 Flavoprotein -.3 Ubiquinol .045 Cytochrome b .077 Cytochrome c1 .22 Cytochrome c .25 Cytochrome a .29 Cytochrome a3 .55 O2 .82 Determined experimentally: Add inhibitors that block e- flow to certain carrier and then look at which carriers are reduced and which are oxidized and do multiple inhibitors to deduce The major complexes (I-IV): what segment of the electron transfer chain does each catalyze? Complex I transfer of e- from NADH to CoQ to make QH2, and this pumps four protons from the matrix into the intermembrane space Complex II not in direct path of e- transfer, but complex II is the enzyme succinate DH, so when it catalyzes reaction of succinate to fumarate, create FADH2, these e- are
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This test prep was uploaded on 04/09/2008 for the course BIOCHEM 507 taught by Professor Nelson&cox during the Fall '08 term at Wisconsin.

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Learning goals for - The endosymbiont hypothesis for the origin of mitochondria Early eukaryotes engulfed a purple bacteria that was capable of

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