week 13 - lactose i Explain the regulation of the lac...

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Study Guide for April 9- April 11 Genetics 2008 Dr. Padilla Study Guide for Week 13. Use to study for exam 3. Topics: Prokarytotic Gene Expression. 1. Define the following: a. Gene expression b. Constitutive expression c. Operon d. Adenyl cyclase e. Isopropyl thiogalactoside (IPTG) f. Repressible gene system g. Inducible gene system h. Promotor i. Operator j. mRNA stem loop structure (also known as hairpin loop structure) 2. In regards to the lactose (lac) operon: a. Diagram the lac operon b. Explain the function of the genes expressed c. What are the cis- elements involved with the regulation of gene expression of the lac operon? d. What are the trans-elements involved with the regulation of gene expression of the lac operon? e. What role does the lactose have in gene expression of this operon? f. Explain the regulation of the lac operon in the presence of glucose and no lactose. g. Explain the regulation of the lac operon in the absence of glucose and presence of lactose. h. Explain the regulation of the lac operon in the presence of both glucose and
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Unformatted text preview: lactose. i. Explain the regulation of the lac operon if a mutation occurs resulting in the inability of the repressor protein to bind the operator region (irregardless of environment). j. Explain the regulation of the lac operon if a mutation occurs so that the repressor protein is always bound to the operator region (irregardless of environment). k. Why is the finding of the lac operon worthy of winning a Nobel Prize (which Lederberg, Jacob, and L’Woff did in 1965)? 3. In regards to the tryptophan (Trp) operon: a. Explain the regulation of the trp operon when tryptophan is absent. b. Explain the regulation of the trp operon when tryptophan is present. c. Explain how attenuation plays a role in the regulation of the trp operon. d. Explain how the codon sequence UGG plays a role in the regulation of the trp operon....
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This note was uploaded on 04/08/2008 for the course BIOL 3452 taught by Professor Padillia during the Spring '08 term at North Texas.

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