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dna - BY 1116 Biological Sciences I Dr Kathryn Ponnock Week...

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BY 1116 Biological Sciences I Dr. Kathryn Ponnock Week 7 – DNA Structure and Replication I. DNA is the Carrier of Genetic Information A. Frederick Griffith Experiments – 1928 1. Used two strains of pneumococcus bacteria 2. One strain (S) was Smooth, Virulent and Lethal 3. Other strain (R) was Rough, Avirulent and Non-lethal 4. Injected mice with heat-killed virulent S strain and mice did NOT DIE . 5. Injected mice with heat-killed virulent S strain and live avirulent R strains – High proportion of mice DIED 6. Griffith isolated living S strain cells from mice 7. Postulated that heat had killed the S strain but did not destroy the hereditary material. Somehow this material was transferred into the living R cells. 8. Known as Transformation 9. Transformation was permanently heritable. 2. Avery, MacLeod and McCarty Experiments – 1944 1. Chemically identified Griffith’s Transforming factor as DNA 2. Used living R cells and highly purified DNA from S cells to obtain the same result as Griffith 3.
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