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Unformatted text preview: Microbiology 509 Score . I18
24 May 2007; TR 2:30, L. Saville-Rath Name ___.___._—
1. (1 pt.) A student in 509 lab mixed a simulated blood sample with anti-A serum and saw clumping. This
clumping was used as an example of antibody combined with A. comlement C. phagocytes B. inso uble antien D. soluble antigen
2. (1 pt.) A antibiotic kills the microbe whereas a(n) antibiotic only
inhibits its growth. A. alleIOpathic, bactericidal C. bacteriosta ‘ actericidal
B. bacteriostatic, antibiosis D. bactericidal, bacteriostatic 3. (1 pt.) A student was given a plate with a streak of a species of Streptomyces growing down the center of the
plate. The student then made a streak of E. coli perpendicular to the center growth. After incubating at 37°C for
24 hr., NO growth of E. coli was seen. The best explanation for this is that Streptomyces A. formed a symbiotic relationship with E. coli B. parasitized the E. coli cells C. prodgged a virulence factor that killed E. coli (“TIDpr‘roduced an anti IO Ic . corwas susceptible to v “-»~—.. ,, a. . ....- .......__._’/ 4. (1 pl.) Symbiosis is a relationship between different species that live together. if the relationship benefits one
species without benefiting or harming the other, it is considered ‘ArMutuaiism WC}: §ynvergismww_
BTParasitism < D. None of'the abové'“\-
NW” _ _‘ v_ 7%.“ J 5. (1 pt.)What organism benefits in the relationship between Clostridium sporogenes and Staphylococcus
aureus cultured together in T88? ,f—h v - ' s A. C. sporogenes C. Both Aand B (—9
B. S. aureus \DZ'Neither A nor B. 6. (1 pt.)The interaction between the two organisms that cause Vincent’s Angina (Trench Mouth) can be defined as: ,, ,
A. Commensalism (C. Sygergism J B. Mutualism D. Parasitism 7. (1 pt.) Leguminous plants (like soybeans) have nodules which harbor bacteria specialized in A. ammonification entation _
B. denitrification D. ni n ti Liqu 8. (1 pt.) The results of the exercise where plates of E. coli were spread with the dilutions of T4 demonstrated
the A. lysogenic cycle’s slow release time. 8. slow penetration of bacteriophage. Q.§Q§dﬁcit;co£attaehment~duringthe lytlc cycle. ' it of prophagewgeriikﬂ 9. (1 pt.) A student spit into a tube of Snyder test agar. After incubation, the tube turned yellow.
This indicates that the bacteria in the student’s mouth
__A_t9£m9nts¢ mannitol . B. produced acid )
‘C. used'the mixed acid pathway D. used glucose but not lactose 10. (15 pt.) A dilution of bacteriophage is to be made to determine its concentration via enumeration. One of the steps asks you to transfer 0.2 ml from one tube to another. Usin the same assum tions concernin the i ette's accurac as ou did in lab, shade in the 1.0 ml pipet to the right to show how much TOTAL diluent you should take into the pipette. 11. (1 p1) A student inoculates a Mitis-Salivarius agar plate with a oral sample taken with a swab,
After incubation he observes colonies. The microorganism forming the colonies is most likely A. Bacillus spp C. Lactobacil/us s . B. Candida spp. ( EZreétococcus )
12. (1 pt.) Mannitol salt agar is selective-differential. lt is selective because of the addition of to the agar; V ., A. Bismuth C. Mannitol ‘ B. Crystal violet 13. (1 pt.) In lab you observed a demo of macrophages from a mouse. The mouse had been injected with
Staphylococcus aureus. How did the S. aureus get inside the macrophages? A. agglutination C. osmosis B. hemolysis D. ha oc tosis 14. 11 pt.) A species of Serratia was streaked onto a plate of test agar and incubated at 37°C for 24 hr. The
plate was then flooded with HCl and a clear zone was seen around the streak of Serratia. This indicated that Serratia r ,
A. DNase and broke doerEltjAE the test agar ) B. coagua se an ro ﬁown nutrients in the test agar
C. complement and caused lysis of blood cells in the test agar
D. hemolysins and caused lysis of blood cells in the test agar 15. (1 p1.) Which type of hemolysis causes complete lysis of erythrocytes in blood agar? A. alpha C. delta
(rrgg’é‘; D. gamma 16. (1 pt.) Sheep erythrocytes and complement are placed into two agglutination tubes. Anti-sheep erythrocyte
serum is added to one of the tubes. How would you identify the tube to which Anti-sheep erythrocyte serum '
had been added?
A. after centrifuging, a pellet wiﬂbeseenmatjhgrbpjtomgj‘thetube
Tﬁfa drop of solution from the tube on a slide and look fora‘g‘gluttnation. D. look for a green color to form following addition of a substrate to the tube 17. (15 pt.) Observe the diagram below of a disk on a spread plate and measure the zone of inhibition
around the Penicillin (P) disk in the same way you did in lab. Put your measurement in mm below: (Must be
accurate within +/- 2mm) ,5 i 5 mm Gray area represents
6:) bacterial growth ...
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- Spring '07