Microbio 509 - Linda Saville-Rath - Lab Quiz 2

Microbio 509 - Linda Saville-Rath - Lab Quiz 2 - ifxl fir...

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Unformatted text preview: ifxl '\ fir a I I 71" (A .7 Microbiology 509 Score l /18 / Lab Quiz 2 19 Apr. 2007; TR12:30, L. Saville-Rath Name» 1. (1 pt.) What waxy lipid found on the surface of acid-fast bacteria’s cell walls retains the primary stain carbolfuc '? ' ' C. turgitol B. nucleic acid D. volutin 2. (1 pt.) Which of the following describes colony morphology. A. Staphylo C. Bacillus B. Strepto < D. Lobed 7 3. (1 pt.) What is the functional classification of MacConkey’s agar? D. Differential B. Selective We-“ CfGenefal—putcpose flyeflfflenflalc) 4. (1 pt.) What is the selective agent in starch agar? A. Bile Salts C. Neutral Red B. Irgasan rm WWI—a 5. (1 pt.) Organism “X” was inoculated onto starch agar. Following incubation was added to the plate and the entire plate turned blue-black around the growth of “X” indicating that “X” A. acetone-alcohol; produce alpha amylase B. neutral red; did not use starch (Q. ioglne; gid not use Sift—FED) D. io ine; pro use a p a amylase 6. (1 pt.) Which of the following correctly describes the 2-pvlgsue streak as you used it in lab? t—dfirusedwhen gutting 3 different or anisms e aft-3% B. used when producing pure culture from a mixed culture C. one organism used to produce 15‘ phase and second to produce 2"‘1 phase D. flaming between the first and second phase should gp_t be done 7. (1 pt.) Three different smears of the same pathogenic bacterium were stained as follows: SMEAR 1: Endospore stain; result showed red cells and blue—green ovals SMEAR 2: Capsule stain; result was no clearing seen around cells SMEAR 3: Acid fast stain; result showed only blue cells These res ould support the diagnosis of which one of the following pathogens? y lostridium teta ' C. Nocardia sp. vBrhycebacterium-tabereulesis vDsSt-reptwoccms’pneumomae 8. (1 pt.) You are given a saline suspension of a mix of three bacteria and asked to produce a pure culture of one of them. Which one of the following media would NOT be a good one to use? A. Brain Heart Infusion agar C. Nutrient agar CB. MacConkey’s agar D D. Trypticase Soy agar 9. (1 pt.) Organism “X” produces yellow colonies on MacConkey’s agar following incubation. This result indicates that NOTE: More than one answer is correct. Circle ALL the correct answers. . IS an enteric C. ferments lactose B. is gram negativ D. is a coliform h 10. (15 pts.) Match the following as they apply to the 3 stains below. C Endospore stain A. seen only as a DEMO in lab (you did not actually prepare) 5 Capsule stain 8. blood smear technique was used to prepare smear A Acid fast stain C. heat from a boiling water bath was used as mordant Use the following table to answer the questions 11 and 12. (-) indicates no growth, (+) indicates growth Organism MM1 MM1 without glucose TSA BHI A - — + ++ B + . ++ +++ i C _ _ . ‘ ++ 11. (O. 5 pt.) None of the organi ms grew on MM1 without glucose because this medium; r 7;, w‘vj’f/ ‘ , lacked a source of ' (\ jg»:- 1V — - _ A k “My ' V_ ,1: a L is.” X ‘1 , . 12. (1 pt.) According to this data, which organism (A, B, or C) is the LEAST fastidious? 13. (1 pt.) Based on your work with mix 3 in lab, what can cause a change in the colony morphology of Serratia marcesence? may/um 7m amidst (Jidi 14. (1.5 pt.) This 3-phase streak plate was inoculated with one of the mixes you used in lab. Based on the pattern of growth seen on the plate, what is one technique error the that may have been made? Le}; ;,L{g:~5\ ()J\\££ *l-u') “(w-t Vi txxijfijxikfi‘fr W\ {Le Ptfi‘v‘ Foo; mittens (Q15)- 'tfifi-VNAA {K‘s-M Most stir}ch Palm £10.qu $€(,;l’\-ifl, . , a t .d ‘ lav: moat/i lam mowfi Sic-La“ \No 1 stch‘D 15. (1.5 pt.) If you wanted to make a pure culture of the bacteria in the isolated colony marked with an “A; what would you need to do (Detaits NOT necessary)? kac a ‘3» p‘mo‘sfl s‘l’l‘ikK a: WA“ t.“ helm/85mg ‘loté 1mm {bout—v Q:\ov\\: 16. (2 pts.) A student diluted a suspension of Eco/iand plated 1 ml of each dilution. After incubation at 37°C on TSA he obtained the following results. Calculate the cfu/ml of the original suspension. Show your work and write your answer using correct scientific notation! Total Dilution Used Colonies per plate l = 10'2 >300 10'3 10“ 33 (km ...
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This note was uploaded on 04/09/2008 for the course MICRBIOL 509 taught by Professor Lindasaville-rath during the Spring '07 term at Ohio State.

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Microbio 509 - Linda Saville-Rath - Lab Quiz 2 - ifxl fir...

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