MidKey07 - 2/9/07 Bio 98A Midterm Name _ score ) If one...

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2/9/07 Bio 98A Midterm Name __________________________________________ score ------------ ) If one combines 0.1 moles of NaH 2 PO 4 and 0.2 moles of Na 2 HPO 4 in 900 ml of water, the final pH will be ________7.16 or 7.2__________. -------- log R = D , so log (0.2/0.1) = 0.301, The basic form prevails around the pKa of 6.86 So pH = 6.86 + 0.30 = 7.16 ) At pH 6.0, the net charge on the peptide shown below is ____-1.5_______. -------- +0.5 -1 -1 +1 A H M E D -1 ) The a -chain of human hemogobin contains 141 amino acids and the b -chain contains 146 amino acids. A single heme group (including the Fe) has a mol. wt. of 614. What is the estimated molecular weight of a hemoglobin molecule? _________65,596_________________________ grams per mole. -------- 2 alpha chains + 2 beta chains + 4 hemes 2x141x110 + 2x146x110 + 4x614 = 65,596 ) Goat myoglobin is 2/3 saturated with O 2 at P O 2 = 60 mm Hg. The P 50 of this myoglobin is __________________30 - 31________ mm Hg. -------- PO 2 2 60 Q = -------- ----- = ------- P 50 + PO 2 , 3 P 50 + 60 2P 50 + 120 = 180, P 50 = 30
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2/9/07 Bio 98A Midterm Name __________________________________________ score ------------ ) The table below shows kinetic data for a reaction catalyzed by 0.01 μM enzyme. From these data, estimate the enzyme's V max , K m , and k cat . V max = ________________70.0_________ μM/min -------- K m = _________________2.0_________ mM -------- ) 30 μg of pure enzyme X (mol. wt. = 60,000) can catalyze the formation of 4.5 μmoles of product per minute when saturated with substrate. The k cat (turnover #) of X is _______________9,000__________ min -1 --------
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MidKey07 - 2/9/07 Bio 98A Midterm Name _ score ) If one...

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