Cap which as you know is a transcription activator

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Chapter 15 / Exercise 9
Biology
Martin/Solomon
Expert Verified
5b) Mutant acontains in the araoperona loss-of-function mutation that prevents the araoperon from being transcriptionally activated by the absence of glucose. List two cis-elementsin the araoperon that could have been mutated. (4)
5c) Mutant balso contains a loss-of-function mutation that prevents transcriptional activation of the operon by the absence of glucose, but the mutation is in a gene outsidethe araoperon. List two different genesthat could have acquired loss-of-function mutations. (4)ara
In the lane on the right labeled 5e, draw the result of the DNase footprint assay if you forgot to add DNase. (2)5d) The araoperon is transcribed only in the presence of arabinose. Based on the footprinting assay, list a key differencein how AraC is regulated by arabinose as compared to how the lac repressor is regulated by lactose. (2)Name:____________________Page 660 n50 n40 n30 n20 n10 n
+ 1 n
70 n
Arabinose: - - + 5e5f5f) TheDNA in the shown DNase footprinting assay is 32labeled at the -50 position. In the lane labeled 5f, draw the result of the DNase footprinting assay if you labeled the DNA at position +20instead of -50. (2)P-
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Biology
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Chapter 15 / Exercise 9
Biology
Martin/Solomon
Expert Verified
Below is shown a genomic region. For the shown genes, exons are shown as white bars and ‘ ‘ indicate +1 positions. Above the genomic region are shown the results of Chromatin immunoprecipitation (ChIP) assays in muscle cells for the indicated chromatin modifications (High: strong ChIP signal; Low: weak ChIP signal).HighMedLowChIPassays (in muscle cells)Based on the results of the ChIP assays below:6b) Box the genomic region predicted to be bound by heterochromatin factor HP1(a chromodomain protein). (2)6c) Use arrow(s) labeled ‘6c’ to indicate the position(s)in the genomic region where RNA polymerase II transcription initiationtakes place in muscle cells. (2)Question 6. ChromatinH3K9-Me3HighMedLowDeletions:iiiiiiivv6d) For deletions i-vi indicated above, based on the positionand the effectof the deletion, list the most likely cis-elementthat has been deleted. (6)Effectcis-element deletedDeletion i:5S RNA is no longer transcribed________________________________Deletion ii:Gene A is transcribed in all tissuesGene CGene AGene B5S RNA GeneGenomic region H3K9-Ac________________________________Deletion iii:Genes B and C are transcribed in skin, where normally only Gene A is transcribed________________________________Deletion iv:Genes B and C are no longer transcribed________________________________Deletion v: Gene C is no longer transcribed________________________________Deletion vi:Genes B and C nucleosomes become modified with H3K9-Me3________________________________

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