Parp pro apoptotic 2 in normal proliferating

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PARP-pro-apoptotic
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2. In normal proliferating epithelial cells, Ras-mediated signaling results in phosphorylation of several target proteins, and a later transient increase in mRNA levels for gene X. Semi-quantitative RT-PCR was performed for gene X in cells following in induction of Ras signaling, and the results are shown in the following graph. A. Draw the curve you expect to see if the cells were incubated with a non-hydrolyzable analog of GTP. B. Would you expect a similar result if phosphatase activity were inhibited. Explain. C. What would you expect if the cells overexpressed a mutant form of MAP kinase that was unable to dimerize?
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A. Cells treated with a non-hydrolyzable GTP analog will have prolonged signal transduction due to the inability of Ras-GTP to turn off. This will result in a prolonged elevation of the transcription of gene X. B. Cells treated with protein phosphatase inhibitors would also have a prolonged signal as the targets of all of kinases of the pathway (Raf, Mek, and MAPK) would remain phosphorylated. C. Cells overexpressing a mutant form of MAPK that doesn’t dimerize would have no transcription as MAPK would not enter the nucleus and activate transcription factors. A, B C
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