Selective flexibacter medium Bullock et ciL 1986 02 wv tryptone 005 wv yeast

Selective flexibacter medium bullock et cil 1986 02

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Selective flexibacter medium (Bullock et ciL, 1986) 0.2% (w/v) tryptone 0.05% (w/v) yeast extract 0.3% (w/v) gelatin 1.5% (w/v) agar SteriUse at 121°C for 15 min, cool to 45°C and add filter-steriHsed neomycin sulphate (0.0004% w/v). Selective kidney disease medium (SKDM) 1.0% (w/v) tryptone 0.05% (w/v) yeast extract 0.005% (w/v) cycloheximide 1.0% (w/v) agar pH 6.8 SteriUse at 1 2 r c for 15 min, cool to 50°C, add sterile foetal calf serum to 10% (v/v), and filter-sterilised solutions of L-cysteine hydrochloride (0.1% w/v), D- cycloserine (0.00125% w/v), polymyxin B sulphate (0.0025% w/v) and oxoHnic acid (0.00025% w/v).
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Isolation/Detection 181 Semi-defined medium for Renibactevium salmoninarum Amount! 1 ^ Ingredient Preparation 10 g Tryptone 10 ml Mineral salts Contains per 200 ml: EDTA dihydrate (disodium salt), lOOmg; Solution MgCl2, 4 g; CaCl2. 2H2O, 1.4 g; FeCl2. 6H2O, lOOmg; ZnS04.7H20, lOOmg; MnS04.4H2O, lOOmg; CUSO4.5H2O, 50mg; C0CI2.6H2O, 50mg; Na2Mo04.2"H20, 50mg 10 ml Nitrogen Contains per 100 ml: uracil, 50 mg; guanine, 50 mg; compounds adenine, 50 mg; xanthine, 50 mg 1 mg each Vitamins Nicotinic acid, riboflavin, thiamine, calcium pantothenate 2 mg each Vitamins Pyridoxal HCl, pyridoxine HCl (prepared after Rogosa et ai, 1961) 20 ml Buffer Contains per 100 ml: K2HPO4, 15g; KH2PO4, (pH 6.8) 15g; sterilised at 12rc/15min 8 ml Cysteine HCl Prepared immediately prior to addition as a solu- tion (12.5% [w/v]) in N NaOH to give a pH of 6.8; filter-sterilised 2 ml Glucose Prepared as a solution (50% [w/v]) in distilled water; steriHsed at 115°C/20min Note: tryptone, mineral salts solution and nitrogen compounds are added to distilled water, the pH adjusted to 6.8 with N NaOH, and the medium dispensed in 190 ml amounts in 800 ml capacity Erlenmeyer flasks. Sterilisation is at 12rC/ 15 min. After cooHng to ~50°C, the pre-sterilised buffer, glucose, cysteine HCl and vitamin solutions are added to a final volume of 200 ml. Agar to 1 % (w/v) may be incorporated, as necessary. Shotts and Waltman medium for the isolation of Edwardsiella ictaluvi (Shotts and Waltman, 1990) 1% (w/v) tryptone 1 % (w/v) yeast extract 0.125% (w/v) phenylalanine 0.12% (w/v) ferric ammonium citrate 0.0003% (w/v) bromothymol blue 0.1% (w/v) bile salts 1.5% (w/v) agar 980 ml distilled water Dissolve by boiling, cool to 50°C and adjust pH to 7.0, sterihse at 12rC for 15 min, cool to 50°C and add mannitol (filter-steriHsed) to 0.35% (v/v) and colistin sulphate to 10 |ig/ml.
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182 Bacterial Fish Pathogens Skimmed milk agar 0.05% (w/v) yeast extract 1.0% (w/v) skimmed milk powder 1.0% (w/v) agar No. 3 pH 7.2 Sterilise at 115°C/15min. TCY medium (Hikida et ciL, 1979) 0.1% (w/v) casamino acids 0.1% (w/v) tryptone 0.02% (w/v) yeast extract 0.1% (w/v) calcium chloride 1.08% (w/v) magnesium chloride 0.07% (w/v) potassium chloride 3.13% (w/v) sodium chloride pH 7.0-7.2 Thioglycollate broth 0.05% (w/v) L-cystine 0.25% (w/v) sodium chloride 0.5% (w/v) dextrose 0.5% (w/v) yeast extract 1.5% (w/v) pancreatic digest of casein 0.05% (w/v) sodium thioglycollate pH 7.1 SteriHseat 12rc/15min Thiosulphate citrate bile salt sucrose agar (TCBS) 0.5% (w/v) yeast extract 1.0% (w/v) peptone 1.0% (w/v) sodium thiosulphate 1.0% (w/v) sodium citrate 0.8% (w/v) ox bile 2.0% (w/v) sucrose 1.0% (w/v) sodium chloride 0.1% (w/v) ferric citrate 0.004% (w/v) bromothymol blue 0.004% (w/v) thymol blue 1.4% (w/v) agar pH 8.6 After boiling to dissolve the ingredients the medium will not require further sterilisation.
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