3 heat shock for 90 seconds at 42c to create a

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(3) Heat Shock for 90 Seconds at 42°C to create a Thermal Imbalance to sweep the DNA into the E. coli. (4) Recovery for 30 Minutes at 37°C to allow Antibiotic Resistance Genes to be Transcribed and Translated (i.e. to allow the new Phenotype Time to catch-up with the new Genotype). This Experiment involved -- A. • Artificial Competence. • The Plasmid was a ~4 Kbp pBR 322. _____________________________________________________________ B. • Artificial Competence. • The Plasmid was a ~9.5 Kbp F Factor. _______________________________________________________________ C. • Natural Competence. • The Plasmid was a ~28 Kbp R Factor. ______________________________________________________________ D. • Natural Competence. • The DNA was a ~95 Kbp F´ (“F Prime”). 41. Which of the following Pairs of Bacteria would both have an Extrachromosomal (i.e. Cytoplasmic) F Factor? A. F + and F - (F Plus and F Minus) _________________________________________________________________ B. F + and F´ (F Plus and F Prime) _________________________________________________________________ C. F + and Hfr (F Plus and H-F-R) _________________________________________________________________ D. F´ and Hfr (F Prime and H-F-R) STOP! Please check your ScanTron for Evidence of Crass Stupidity. You have just answered Question 41. Proceed to Question 42.
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MIC 101 Exam 1 Page 25 42. In 1955 François Jacob presented a Paper on the Hfr Strains at Cold Spring Harbor. Before leaving the US he bought a Waring Blender for his Wife. The Blender did not find a Home in the French Kitchen: “My wife was completely disgusted with this Instrument.” The Blender was banished to the Lab. Jacob ʼ s Colleague Élie Wollman was aware of Hershey and Chase ʼ s Classic “Waring Blender Experiment” of 1952 and borrowed the Idea of using it for what became known as the “Coitus interruptus” Experiment. Jacob and Wollman used different Hfr Strains of E. coli with F Factors integrated at different Sites (and in different Orientations) to build-up a Temporal Map of the E. coli Chromosome. Theirs was the first Map of a Bacterial Chromosome. It was jumping up and down and doing Handstands trying to tell Researchers that the Bacterial Chromosome was a Circle (Yes, a Circle!). It took several Years before this absolutely Novel Concept was accepted. Which E. coli Strains did Jacob and Wollman use for the very first of the many Interrupted Matings used to generate the first Map of the E. coli Chromosom? A. • The Donor was a Penicillin-Sensitive Hfr Strain: (Hfr Lederberg Bio + Lac + Gal + Pen S ) • The Recipient was a Penicillin-Resistant F´ (“F Prime”) Strain: (F´ Bio - Lac - Gal - Pen R ) ___________________________________________________________ B. • The Donor was an Penicillin-Resistant Hfr Strain: (Hfr Monod Bio + Lac + Gal + Pen R ) • The Recipient was a Penicillin-Sensitive F´ (“F Prime”) Strain: (F - Bio - Lac - Gal - Pen S ) ___________________________________________________________ C. • The Donor was a Streptomycin-Sensitive Hfr Strain: (Hfr Hayes Thr + Leu + Gal + Str S ) • The Recipient was an Streptomycin-Resistant F - Strain: (F - Thr - Leu - Gal - Str R ) ___________________________________________________________ D. • The Donor was a Streptomycin-Resistant Hfr Strain: (Hfr Luria Thr + Leu + Gal + Str R ) • The Recipient was a Streptomycin-Sensitive F - Strain: (F - Thr - Leu - Gal - Str S )
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MIC 101 Exam 1 Page 26 43. Our Evil Scientist approaches Carl from behind with a Microtiter Tube containing Bacteriophage used in her Lab for successful Transduction Experiments.
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