B fake rate graph vi analysis in the enzyme catalysis

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b.Fake Rate Graph VI. Analysis In the enzyme-catalysis lab, the following balanced equation is observed and studied-
2H2O2 +catalase → 2H2O+OThe hydrogen peroxide in this lab acts as the substrate, catalase acts as the enzyme, the intermediate complex formed is hydrogen peroxide catalase, and the products are water, oxygen, and catalase because the enzyme was unchanged. In the beginning of the lab enzyme activity wastested with the materials that were used later on in the lab. 10mL of hydrogen peroxide and 1 mLof catalase was added to a cup. After 30 to 60 seconds, there was a chemical reaction that occurred as the peroxide bubbled and fizzed. This indicated a chemical change because the reaction gave off gas. This established the basis and proved that there was enzyme activity allowing the lab to continue. The effect of boiling on enzyme activity was then observed as 3-5 mL of catalase was added to a test tube and the test tube was placed in boiling water for five minutes. 10 mL of hydrogen peroxide was then transferred to a cup and 1mL of the boiled catalase was added into the same cup. Boiling the catalase impacted its activity because the catalase does not bubble, therefore showing the reaction did not take place. The enzyme was ultimately denatured at the tertiary and quaternary levels. There are many factors that could also impact the activity of the catalase besides boiling. Temperature could also impact the activity of the catalase because the catalase has an optimal temperature that it works best at in the body. If the body gets too hot, the catalase could be denatured, just like in the boiling situation. pH could also impact the activity ofthe catalase because catalase also has an optimal temperature that it works best at. If the pH is too acidic or too alkaline, it could prohibit the catalase from working properly. If it is not in closerange of the optimal pH, the activity could slow down tremendously. Substrate concentration could also impact the activity of catalase. If there is no substrate for the catalase to act upon, no activity can be done. If the substrate concentration is elevated, the catalase will work at a faster
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pace because it is easier to find something to work with. Enzyme concentration can also impact the activity of an enzyme. If there is a small amount of enzyme, the reaction will proceed slower because there is not enough enzyme for every substrate to be acted upon. If the concentration of the enzyme is high, the reaction will proceed at a much quicker pace.

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