A polymerase chain reaction kcr is a laboratory

  • No School
  • AA 1
  • 4

This preview shows page 2 - 4 out of 4 pages.

patients. A polymerase chain reaction (KCR) is a laboratory method to amplify targeted fragments in a DNA molecule. Quantitative PCR (QPCR) is a laboratory method that uses the basic principles of PCR, but occurs in real time and allows for the DNA quantification (Porcher et al., 1992). The real-time detection is facilitated by a fluorescent dye that is incorporated into the minor grooves of a double-stranded DNA molecule AqPCR goes through two phases, an
Image of page 2

Subscribe to view the full document.

exponential phase when the PCR reaction is not limited by reagents and a non-exponential plate phase, which occurs when reagents are consumed and one or more rent become limiting. An amplification plot is produced during the course of a qPCR (see graph), which charts the amount of florescence ( s) versus the current PCR cycle s ). The preenline represents the fluorescent values collected along the cycle. The threshold line represents background levels of fluorescence. The threshold cycle (G) value is the cycle during which the florescence is due to sample based differences and not solely the background. The value is then used to calculate relative or solute values of gene expression - + + Quantitative PCR data are used to calculate rates of gene expression. The relative amounts of gene expression are calculated by first normalizing the qPCR results by using both treated and untreated samples of target and reference sequence. This is done using the Livak method, or AAC method (Livak and Schmittgen, 2001). AG - C wm -IG - Go ,
Image of page 3
Image of page 4

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Ask Expert Tutors You can ask 0 bonus questions You can ask 0 questions (0 expire soon) You can ask 0 questions (will expire )
Answers in as fast as 15 minutes