Following transfection the culture will include three

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Following transfection, the culture will include three types of cells: A) cells that have lost the transgene without inserting it B) cells that have inserted the transgene by homologous recombination at the FGFR3 locus C) cells that have inserted the transgene at another locus by end-joining to a nicked strand followed by DNA repair. TK Neo r FGFR3 FGFR3 Vector Vector Clicker question In medium containing gancyclovir alone, the most frequent class of cells will be ___ (select A, B, or C).
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TK Neo r FGFR3 FGFR3 Vector Vector A) cells that have lost the transgene without inserting it B) cells that have inserted the transgene by homologous recombination at the FGFR3 locus C) cells that have inserted the transgene at another locus by end-joining to a nicked strand followed by DNA repair. Clicker question: In medium containing neomycin alone, the most frequent class of cells will be ___ .
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TK Neo r FGFR3 FGFR3 Vector Vector A) cells that have lost the transgene without inserting it B) cells that have inserted the transgene by homologous recombination at the FGFR3 locus C) cells that have inserted the transgene at another locus by end-joining to a nicked strand followed by DNA repair. In medium containing neomycin and gancyclovir, the most frequent class will be ___.
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Molecular Biology of the Cell NBK26818/#A1654 Next steps Bred with white (recessive) mice We are here
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Figure 22-91 Molecular Biology of the Cell (© Garland Science 2008) ES cells with gene knockout or knock-in Step 5: Introducing the
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