Lecture5genetargeting

Http wwwncbinlmnihgov booksnbk21632a1984 you

Info iconThis preview shows pages 13–18. Sign up to view the full content.

View Full Document Right Arrow Icon
http:// www.ncbi.nlm.nih.gov/ books/NBK21632/#A1984
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
You transfect ES cells from a brown mouse (dominant marker) with this targeting vector: Following transfection, the culture will include three types of cells: A) cells that have lost the transgene without inserting it B) cells that have inserted the transgene by homologous recombination at the FGFR3 locus C) cells that have inserted the transgene at another locus by end-joining to a nicked strand followed by DNA repair. TK Neo r FGFR3 FGFR3 Vector Vector Clicker question In medium containing gancyclovir alone, the most frequent class of cells will be ___ (select A, B, or C).
Background image of page 14
TK Neo r FGFR3 FGFR3 Vector Vector A) cells that have lost the transgene without inserting it B) cells that have inserted the transgene by homologous recombination at the FGFR3 locus C) cells that have inserted the transgene at another locus by end-joining to a nicked strand followed by DNA repair. Clicker question: In medium containing neomycin alone, the most frequent class of cells will be ___ .
Background image of page 15

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
TK Neo r FGFR3 FGFR3 Vector Vector A) cells that have lost the transgene without inserting it B) cells that have inserted the transgene by homologous recombination at the FGFR3 locus C) cells that have inserted the transgene at another locus by end-joining to a nicked strand followed by DNA repair. In medium containing neomycin and gancyclovir, the most frequent class will be ___.
Background image of page 16
Molecular Biology of the Cell http://www.ncbi.nlm.nih.gov/books/ NBK26818/#A1654 Next steps Bred with white (recessive) mice We are here
Background image of page 17

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 18
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page13 / 26

http wwwncbinlmnihgov booksNBK21632A1984 You transfect ES...

This preview shows document pages 13 - 18. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online