Part 2: Fluorescence of riboflavin Goal Divide work amongst your group to create a calibration curve for riboflavin. Use the calibration curve to determine the concentration of riboflavin in Rockstar energy drink. Procedure 1.Obtain approximately 50 mL of riboflavin (approximately 25.00 mg/L). Record the exact concentration of the stock solution. You will need to create dilutions of this stock solution in order to measure the constant kin ࠵? =࠵?࠵?8࠵?. Buret and Flask Group work: Student #3 - Riboflavin 1.Using the buret and flask method (Appendix A), prepare one standard solution at a time, transferring each solution to a cuvette for analysis. Use the table belowas a guide for solution preparations. Use %1 acetic acid to dilute your solutions to total volume of 5-mL. 2.Record the exact volumes you actually dispense and use these to calculate your final riboflavin concentration. When reusing the same glassware or cuvettes, it is good analytical practice to proceed from low to high concentration. Why? Table 4.Riboflavin standards preparation Standard Initial riboflavin concentration (M) Target volume of riboflavin (mL) Actual volume of riboflavin dispensed (mL) Total volume (mL) Final riboflavin concentration (M) 9 1.0 5.00 10 2.0 5.00 11 3.0 5.00 12 4.0 5.00 13 5.0 5.00 14 6.0 5.00 15 7.0 5.00 16 8.0 5.00 3.Using a fluorescence spectrophotometer, measure the intensity versus wavelength (Appendix C) for each standard solution.