4 raise your hand when you are ready for your ta to

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4. Raise your Hand when you are ready for your TA to evaluate your Microscope Alignment. 5. After your TA has evaluated your Microscope Alignment, proceed with the Microscope Nomenclature Portion of the Practical. Microscope Nomenclature (5 x 2 Points) 1. There are four Microscopes set-up on the Benchs in SLB 2083 (the Prep Room). Two Microscopes have five (5) Parts labeled A, B, C, D and E; the other two have five (5) Parts labeled 1, 2, 3, 4 and 5. 2. Circle whether the Parts you are identifying are labeled with Letters or Numbers. If you do not circle Letters or Numbers on your Answer Sheet you will be docked two (2) Points. 3. Write the Name of the Part to which the Label is attached in the appropriate Space on your Answer Sheet. For Example, if the Label “3” is on the Eyepiece, write “Eyepiece” in the Space next to C/3 on your Answer Sheet. 4. When you have finished, please put your Answer Sheet in the Box by the Exit to the Hallway. A Copy showing what the Microscope Practical Answer Sheet looks like is on Page 5
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Lab 9 Page 3 Staining Practical Please put your Notes away during the Staining Practical. Gram Staining (2 x 5 Points) 1. There are two Sets of Unknowns in each Lab. One Set consists of Bacterium A and Bacterium B; the other Set consists of Bacterium 1 and Bacterium 2. 2. Indicate on your Answer Sheet whether the Unknown Bacteria you are Gram Staining are labeled with Letters or Numbers. 3. Make a Gram Stain of each of the two (2) Unknown Bacteria provided in Plates on the Back Bench. You should use a Gram Positive Control and a Gram Negative Control on each of your Unknown Gram Stain Slides. 4. Indicate on the Answer Sheet whether each is Gram Positive or Gram Negative You could have two Gram Positive Unknowns, two Gram Negative Unknowns or one Gram Positive Unknown and one Gram Negative Unknown. The Gram Stain Unknowns are Randomized. 5. Please feel free to make multiple Gram Stains of any Unknown to Double-Check these Gram Stain Reactions. 6. Please turn in your Answer Sheet before you leave the Lab. Negative Staining (5 Points) 1. Make a Negative Stain using our Azomonas Culture. 2. Once you have your Negative Stain Slide focused on the Azomonas Capsule at 40X, have your TA evaluate your Slide.
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