Amylase activity 5 mg 1243 mins 00261 gml 15414

This preview shows page 2 - 3 out of 3 pages.

3.A) One of the aims of this experiment was to ‘interpret results in terms of the metabolic role of amylase during plant development’; how well do you think this experiment fulfilled this aim? Explain your answer, including at least one strength and one weakness in theexperiment. B) How would you improve this experiment to better align with the experimental aims?The aim was to analyse amylase activity in dormant, germinating and whole barley seedlings. Our results showed that germinating seeds have the highest amylase activity and it reached achromic point fastest, this is because amylase is involved in metabolizing starch into sugar for the embryo to grow. The seedlings had the second highest amylase activity as they do not require as much starch as the germinating seeds because they are also photosynthesizing which aids in their development and therefore don’t rely on starch for energy (Ernst, 1971). No activity was recorded for the dormant seeds as it is dormant and does not require starch (Biswas et al. 1978). The experiment fulfilled the aim quite accurately as the use of iodine was effective because it is specific on one substance only, starch, and showed a visible colour change (yellow to blue-black) in the presence of starch. Another strength is that as a group, we gathered all our data together to obtain a more reliable result. However, a weakness in the experiment was the amount of iodine added into the wells. Although using

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture