{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lucci_Prelab8_Monday2pm

The greater the solubility in the mobile organic

Info iconThis preview shows pages 2–4. Sign up to view the full content.

View Full Document Right Arrow Icon
The greater the solubility in the mobile organic phase (relative to its solubility in the stationary phase), the greater its tendency to remain in the organic phase and the farther the ascent in the solvent rises by capillary action. 12. List the two organic solvents and the mix proportion used as the solvent (mobile phase) to separate the plant pigments. 1mL Acetone 3mL Petroleum Ether 13. List the pigment name and color in the order they should separate. 1) Carotenes (pro-vitamin A) [orange-yellow] 2) Chlorophyll a [blue-green] 3) Chlorophyll b [yellow-green] 4) Xanthophylls [bright yellow] Exercise 8.4: 14. Describe the technique of paper chromatography. In what ways is it different than TLC? The pigments will be placed 2cm from the bottom with the solvent, just as done for a TLC. However, we will be watching for bands of color and recording how fast the bands of color move in comparison with the solvent. 2
Background image of page 2

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
15. List the pigment name and color in the order they should separate with paper chromatography. 1) Carotenes (pro-vitamin A) [yellow] 2) Xanthophylls [yellow] 3) Chlorophyll a [blue-green] 4) Chlorophyll b [yellow-green] 16. What three factors determine the differential movement of pigments in paper chromatography? 17. What does R f refer to? The relationship of the distance moved by the pigment to the distance moved by the solvent. 18.
Background image of page 3
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page2 / 4

The greater the solubility in the mobile organic phase...

This preview shows document pages 2 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon bookmark
Ask a homework question - tutors are online