Lucci_Prelab8_Monday2pm

Stationary phase the greater its tendency to remain

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stationary phase), the greater its tendency to remain in the organic phase and the farther the ascent in the solvent rises by capillary action. 12. List the two organic solvents and the mix proportion used as the solvent (mobile phase) to separate the plant pigments. 1mL Acetone 3mL Petroleum Ether 13. List the pigment name and color in the order they should separate. 1) Carotenes (pro-vitamin A) [orange-yellow] 2) Chlorophyll a [blue-green] 3) Chlorophyll b [yellow-green] 4) Xanthophylls [bright yellow] Exercise 8.4: 14. Describe the technique of paper chromatography. In what ways is it different than TLC? The pigments will be placed 2cm from the bottom with the solvent, just as done for a TLC. However, we will be watching for bands of color and recording how fast the bands of color move in comparison with the solvent. 2
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15. List the pigment name and color in the order they should separate with paper chromatography. 1) Carotenes (pro-vitamin A) [yellow] 2) Xanthophylls [yellow] 3) Chlorophyll a [blue-green] 4) Chlorophyll b [yellow-green] 16. What three factors determine the differential movement of pigments in paper chromatography? Molecular size, polarity, & solubility 17. What does R f refer to? The relationship of the distance moved by the pigment to the distance moved by the solvent. 18. List the two organic solvents and the mix proportion used as the solvent (mobile phase) to separate the plant pigments.
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