Question 3 a there are a set of enzymes called

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Question 3 a) There are a set of enzymes called phospholipases, which cleave phospholipids at specific bonds. What are some of their in vivo functions? 3b) Describe an experiment in which phospholipases can be used as analytical tools to determine if a specific phospholipid is asymmetrically distributed in the two leaflets of the plasma membrane. 3c) What are some examples of phospholipids that are asymmetrically localized? 3d) What are reasons for this asymmetry?
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Question 4 a)Your lab has produced a mouse which is a knockout for the LNO gene. This gene is a known receptor for the SVU growth factor. This is an important receptor during development and loss of this gene causes embryonic lethality in your mouse. The embryos do survive for 14 days in utero. In order to study the loss of this gene, you decide to create a cell line from the mouse embryos. How would you do this? 4b)You want to determine the specific binding of the SVU to the LNO receptor to find the Kd of the receptor and the number of receptors per cell. How would you do this? What would be your controls? 4c)Surprisingly, you get similar binding curves for cell lines expressing the LNO receptor and the cell line from your knockout mouse. You are worried your mouse was not really a protein null! How could you test for presence of the LNO receptor in the knockout cell line?
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